Lie KK, Meier S, Olsvik PA. 2009. Effects of environmental
relevant doses of pollutants from offshore oil production on Atlantic
cod (Gadus morhua). Comp Biochem Physiol C Toxicol Pharmacol.
2009 Apr 17. [Epub ahead of print]
Lie KK, Lanzen A, Breilid H, Olsvik PA. 2009. Gene Expression Profiling
in Atlantic Cod (Gadus morhua L.) from Two Contaminated Sites
Using a Custom Made cDNA Microarray. Environ Toxicol Chem. 2009 Apr 13:1.
[Epub ahead of print]
Olsvik PA, Lie KK, Sturve J, Hasselberg L, Andersen OK. 2009. Transcriptional
effects of nonylphenol, bisphenol A and PBDE-47 in liver of juvenile Atlantic
cod (Gadus morhua). Chemosphere. 2009 Apr;75(3):360-7. Epub 2009
Jan 22.
Saele Ø, Nordgreen A, Hamre K, Olsvik PA. 2009. Evaluation of candidate
reference genes in Q-PCR studies of Atlantic cod (Gadus morhua)
ontogeny, with emphasis on the gastrointestinal tract. Comp Biochem Physiol
B Biochem Mol Biol. 2009 Jan;152(1):94-101. Epub 2008 Oct 14.
Olsvik PA, Lie KK, Goksøyr A, Midtun T, Frantzen S, Maage A. 2009.
Are Atlantic cod in Store Lungegårdsvann, a seawater recipient in
Bergen, affected by environmental contaminants? A qRT-PCR survey. J Toxicol
Environ Health A. 2009;72(3-4):140-54.
Olsvik, P.A., Lie, K.K., Sturve, J., Hasselberg, L. & Andersen, O.-K.
2008. Transcriptional effects of nonylphenol, bisphenol A and PBDE-47
in juvenile Atlantic cod Gadus morhua. SETAC World, 3-7 August,
Sydney, Australia.
Olsvik, P.A., Lie, K.K., Goksøyr, A. 2008. Are Atlantic cod in
Store Lungegårdsvann, a seawater recipient in Bergen, affected by
pharmaceuticals from sewage effluents? A qPCR survey. 2nd Norwegian Environmental
Toxicology Symposium, 2.-4. April, Trondheim, Norway.
Olsvik PA, Søfteland L, Lie KK. 2008. Selection of reference genes
for qRT-PCR examination of wild populations of Atlantic cod Gadus
morhua.
BMC Res Notes. 2008 Jul 16;1:47.
Lie, K.K. & Olsvik, P.A. 2008. Bruk av cDNA mikromatrise til identifisering
av genuttrykk-profiler hos torsk (Gadus morhua) fra to områder med
forhøyet innhold av miljøgifter. Havbruk 2008, 7.-9. April
2008, Tromsø, Norway.
Olsvik, P.A. & Lie, K.K. 2007. Toxicogenomic tools for Atlantic cod
(Gadus morhua). PRIMO 14, 2007. 6th – 9th May, Florianópolis,
Brazil.
39.
Lie, K.K. & Olsvik, P.A. 2007. Gene expression profiling in four
Atlantic cod populations. PRIMO 14, 2007. 6th – 9th May, Florianópolis,
Brazil.
Olsvik, P.A., Kristensen, T., Waagbo, R., Tollefesen, K.-E., Rosseland,
B.O. & Toften, H. 2006. Effects of hypo- and hyperoxia on transcription
levels of five stress genes and the glutathione system in liver of Atlantic
cod Gadus morhua. Journal of Experimental Biology, 209, 2893-2901.
Olsvik, P.A., Lie, K.K., Stavrum, A.-K. & Meier, S. 2005. Gene expression
profiling of zebrafish exposed to produced water. 8th International Meeting
of the Microarray Gene Expression Data Society (MGED8), 11-13 September
2005, Bergen, Norway (on the microarray technique).
Olsvik, P.A. 2005. Nutri-toxicogenomic studies in Atlantic cod - Current
and planned research at the National Institute of Nutrition and Seafood
Research. Workshop on Genetic research in Atlantic cod - Current status
and future aspects. 10-11th May 2005, Reykjavik, Iceland.
Olsvik, P.A., Meier, S., Andersen, R.A., Arukwe, A., Celander, M., Goksøyr,
A., Hylland, K., Waagbø, R. & Hemre, G.I. 2004. Developing
new tools for genomic research in the Atlantic cod. Environmental Toxicology
Symposium, 19-20 October 2004, Adamstuen, Oslo, Norway.
Kai K. Lie, Rune Waagbø, Gro-Ingunn Hemre, Ivar Hordvik, Sonnich
Meier, Anders Goksøyr, Ketil Hylland, Augustine Arukwe, Rolf A.
Andersen, Malin Celander & Pål A. Olsvik, 2006. Effects
of environmental and nutritional stress on gene regulation in Atlantic
cod Gadus morhua. HAVBRUK 2006 Conference. Bergen, 29
– 31 March 2006.
Abstract:
The main goal of this project is to develop a custom-made microarray
platform for gene regulation studies in the Atlantic cod. A small-scale
oligo-based array with 150-300 sequences encoding proteins involved
in responses to various forms of stress is planned made. Focus will
be put on the parts of the transcriptome that are involved in the
cellular stress response and other regular stress genes, in addition
to a number of housekeeping and control genes. To find the genes
of interest, cDNA libraries will be made from tissues of fish exposed
to waterborne pollutants and dietary undesirables and supplements.
EST’s will be sequenced and assembled,
and used to construct oligos. This project is running from June 2005
to May 2008. A PhD student was hired from 1/6-2005. The project operates
as collaboration between several research groups that work on various
aspects of stress in fish. Knowledge gained by this project will
be of importance for the establishment of a successful cod aquaculture
industry, providing biomarker tools in assessing normal status and
early signs of adverse fish health, and help pave the way for proteomics.
It will also help establish the Atlantic cod as a central biomonitoring
fish species in marine ecosystems in Norwegian waters.
So far about 4800 EST clones have been sequenced from three cDNA libraries
made from liver of Atlantic cod exposed to various pollutants, in collaboration
with the ongoing project “Stress genes in the Atlantic Cod Gadus
morhua: EST sequencing and microarray production” (NFR project no.:
159197/I20). In collaboration with the Computational Biology Unit (FUGE
platform) at the University of Bergen, a pipeline for automatic gene annotation
has been established. From one cDNA library from cod force fed a mixture
of toxicants (i.e. PAH, PCB, Cu and Cd), approximately 500 contigs and
1400 singlets were found, representing about 1900 genuine gene sequences.
In addition, 10000 EST’s have been ordered from a company in China,
from two cDNA libraries; one from gill tissue and one from head kidney
tissue. Many of the stress genes planned
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